Fig. 3. The relationship between CEP110 and Cep250/c-Nap1. HeLa centrosomes were triple-stained with the autoimmune serum M4491 (red), the anti-CEP110 antibody (purple) and an antibody against the centriolar protein Cep250/c-Nap1 (green). (a) Centrosome in the process of duplication. (b) Centrosomes after duplication and separation. The diagrams on the left illustrate the views of the centrosomes seen in the images. The arrow denotes the co-localization of CEP110 and Cep250/c-Nap1 at the daughter centrosomal bud (a) or the well-formed daughter centrosome tube (b). The arrowhead points to the co-localization of the two proteins at the mother centrosomes when centrosome duplication is still going on (a) or after duplication and separation (b). Bar, 5 µM.