Fig. 4. The distribution of ninein within the centrosome tube. The centrosomes were stained with the autoimmune serum M4491 (red) and the anti-ninein antibody (green). The diagrams on the left illustrate the views of the centrosomes seen in the images. (a,b) Centrosomes before duplication. (c,d) The centrosomes in the process of duplication. (e) The centrosomes after duplication and separation. The arrow points to the mother centrosome when the centrosome is in the process of duplication (c,d) or duplication is completed (e). The arrowhead denotes the daughter centrosomal bud (c,d) or the well-formed daughter centrosome (e). Bar, 5 µM.