Fig. 9. Loss of centrosome MTOC function in post-mitotic PtK2 cells injected with anti-ninein antibodies. Control, cell injected with normal serum (a,b); ninein, cell injected with anti-ninein antibodies (c-f). (a,c,e) The injected cells revealed by staining with secondary antibodies. (b,d) The injected cells were stained with anti-ß-tubulin antibodies to illustrate microtubules. The arrowhead points to microtubules nucleated from the centrosome in the control cell. (f) The injected cell was stained with M4491 for the centrosome. The arrow points to the centrosome. (Inset) Enlarged view of the centrosome. Bar, 10 µM.