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Fig. 3. Growth behavior of pub1 and pub2 disruptants. (A) pH sensitivity of leucine auxotrophic pub+ (MM71-6B), pub1{Delta} (KKT81-3A) and pub2{Delta} (KKT85-8A) strains. These strains were grown for 5 days on EMM2 media at the indicated pH. (B) Colony formation at different incubation temperatures. L972 (wildtype), KKT83-9A (pub1{Delta}), KKT39-7C (pub2{Delta}) and KKT82-2D (pub1{Delta} pub2{Delta}) were incubated on YEA medium at 30°C, 34°C, 35.5°C or 37°C.