Fig. 4. DdNek2 resides at the centrosome throughout the entire cell cycle. Fluorescence microscopy of Dictyostelium AX2 cells labeled with anti-DdCP224 antibodies (A-A'') and GFP-DdNek2 cells (B-B''). Cells were fixed with methanol. The secondary antibody in (A-A'') was Cy3-labeled anti-mouse IgG. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI). Bar, 2 µm.