Fig. 5. Overview of the phenotypes of GFP-DdNek2 (A-E) and GFP-K33R (F-J) mutants. Immunofluorescence microscopy of cells fixed with methanol and labeled with anti--tubulin (A'-J'). The secondary antibody was Alexa-568-labeled anti-rabbit IgG. Nuclei were stained with DAPI. The main phenotypes shown are supernumerary MTOCs (A,B,F,G,H,E,J), nuclear aberrations (C,H,J), misshapen centrosomes (D,I) and multiple GFP foci (E,J). Bar, 2 µm.