Fig. 7. Centrosomal localization and phenotypes of GFP-DdNek2 deletion mutants. Two representative examples each are shown for GFP-267-N (A,B), GFP-315-N (C,D), GFP-258-C (E,F) and GFP-312-C (G,H) mutants. Cells were fixed with methanol and labeled with anti--tubulin. The secondary antibody was Alexa-568-labeled anti-rabbit IgG. Nuclei were stained with DAPI. Bar, 2 µm.