Fig. 1. Plasmid for expression of mitochondria-targeted GFP in Neurospora crassa. (A) Structure of the mtGFP cassette. The sequence coding for GFP including a stop codon is symbolised by a white box, regulatory sequences of the atp-1 gene are depicted in grey, introns are delineated by black lines, and regions coding for parts of the F₁ protein are depicted by black boxes. F₁ pre, region of the atp-1 gene coding for the mitochondrial presequence; F₁C, region of the atp-1 gene coding for the C-terminus of the F₁ protein (not translated in the chimeric construct); atp-1 ter., atp-1 terminator. (B) Plasmid map for pNc-mtGFP. All relevant functional elements are indicated. The ampicillin resistance gene (amp^r) and the origin of replication for E. coli (ColE1 ori) are derived from the pBluescript vector. The plasmid map is not drawn to scale. Unique restriction sites are underlined. See text for cloning details.