Fig. 2. Intracellular localization of F-actin and myc epitope in wild-type cells, and cells expressing 34 kDa-myc and CT-myc proteins. Wild-type AX-2 (A), 34 kDa-myc- (B) and CT-myc (C)-expressing cells were observed by DIC (left), and stained with rhodamine-labelled phalloidin (middle) and antibody to the myc-epitope tag (right). There is extensive cortical staining of F-actin in the AX-2 and 34 kDa-myc cells (A,B). By contrast, the CT-myc cells reveal ellipsoids that label strongly for actin and for the myc epitope (C). The asterisks indicate the ellipsoids. Bar, 10 µm.