Fig. 3. Intracellular localization of F-actin, 34 kDa protein, and myc epitope tag in CT-myc cells. CT-myc cells were viewed by DIC microscopy (A,B), stained with phalloidin to localize F-actin (C,D), and stained with antibody to localize the 34 kDa protein (F), or myc epitope tag (E). F-actin and the 34 kDa protein epitopes are present in both the ellipsoids and the cortical regions of the CT-myc cells. By contrast, the myc epitope is largely confined to the ellipsoids in CT-myc cells. Bar, 10 µm.