Fig. 2. Northern blot analysis of HS core protein and FGF receptor expression. Total RNA blots of CMEC and HUVEC cultured under hypoxic (+) or normoxic (-) conditions probed for expression of syndecans 1,2 and 4, glypican-1, perlecan and FGF receptor 1 (FGF R1). Ethidium bromide staining of the 18S RNA band is shown as the loading control. Note the decreased expression of syndecan-1, 4 and glypican-1 under hypoxic conditions and the lack of significant change in expression with other tested genes.