Fig. 1. Time-dependent increase in DNA synthesis in cultured human vascular SMC stimulated with PDGF or bFGF. Quiescent cells in 96-well plates were treated with 1% FCS ([UNK]); 1% FCS+10 ng/ml bFGF (); or 1% FCS+20 ng/ml PDGF (). Cells were exposed to 4x12 hour BrdU pulses (10 µM) from 0-48 hours in the continuous presence of the growth factors. BrdU uptake was determined by ELISA. Data points represent absorbance levels (medians and IQR, n=6).