Fig. 3. UPA-mediated DNA synthesis in PDGF- or bFGF-stimulated vascular SMC. Growth arrested cells were exposed to PDGF or bFGF (for 48 hours) in the absence or presence of polyclonal goat anti-UPA IgG (anti-UPA; 40 µg/ml) or goat nonimmune IgG (goat-IgG; 40 µg/ml). The BrdU incorporation during the second 24 hours incubation period was determined by ELISA. The BrdU-labeling index was calculated as the ratio of the corresponding value in 10% FCS-stimulated cells. Results, given as box-and-whisker plots, were obtained from triplicate determinations in five independent experiments. The Mann-Whitney test was employed to identify differences between groups (PDGF stimulation: anti-UPA, P=0.004; goat-IgG, P>0.05 versus vehicle; bFGF stimulation: anti-UPA, P=0.004; goat-IgG, P>0.05 versus vehicle). The control group represents cells treated with 1% FCS alone (PDGF plus vehicle versus control P<0.0001; bFGF plus vehicle versus control P<0.0001).