Fig. 11. The effect of CaM antagonists on CaM phosphorylation. CaM antagonists melittin or calmidazolium were added to a reaction mixture of CaM phosphorylation by 20 units of CaPK-2 (in the presence of 13.5 µM free Ca²⁺; CaPK-2 is half-saturated at 0.2 µM free Ca²⁺). (A) Lane 1, control — no antagonist; lane 2, 0.5 µM; lane 3, 2 µM; lane 4, 5 µM; lane 5, 10 µM melittin. (B) Lane 1, 0.5 µM; lane 2, 2 µM; lane 3, 5 µM; lane 4, 10 µM calmidazolium.