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Fig. 3. Immunoblot analysis of (A) antibody specificity and (B) immunocrossreactivity of PCBP-25{alpha} and PCBP-25ß. Samples were subjected to SDS-PAGE, transferred to nitrocellulose and probed with either polyclonal antibody against Chlamydomonas centrin or with monoclonal antibody 7A9 as indicated. (A) Lane C, Chlamydomonas whole cell, 15 µg; lane P, quick-killed Paramecium, 20 µg. (B) MonoQ fractions (20 µl) containing PCBP-25. Fraction numbers are indicated at the bottom of the blot. M, Mr of markers (x103).