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Fig. 4. Immunodetection of PCBP-25 in subciliary fractions. Subciliary fractions were generated as described in the Materials and Methods. (A) Coomassie-stained SDS-PAGE, (B) immunoblots with antibody against centrin and (C) with monoclonal antibody 7A9. The same amount of protein (15 µg) was loaded in each lane. Lane 1, whole cilia; lane 2, Triton-extracted ciliary membrane; lane 3, axoneme; lane 4, dynein fraction released by high salt from axoneme; lane 5, axoneme after high-salt extraction. M, Mr of markers (x103).