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Fig. 5. Immunodetection of PCBP-25 in purified dynein fractions. Paramecium 22S and 12S dyneins were purified by sucrose gradient sedimentation from the high-salt extract (0.6 M KCl) of demembranated axonemes. Ponceau S-stained SDS-PAGE (A), immunoblots with antiserum against centrin or with monoclonal antibody 7A9 (B). The same amount of protein (10 µg) was loaded in each lane. Lane 1, 22S dynein; lane 2, 12S dynein; M, Mr of markers (x103).