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Fig. 7. EM localization of PCBP-25{alpha} and PCBP-25ß with gold-conjugated antibodies. (A-C) Using a polyclonal antibody against Chlamydomonas centrin, PCBP-25{alpha} was localised in the transition zone of the ciliary basal body (A) and in the ICL (B,C). (D-F) Using monoclonal antibody 7A9, PCBP-25ß was localised in the ICL (D,E) but not in the basal body (D,F). a, alveoli; ax, axoneme; bb, basal body; c, cilium; icl, infraciliary lattice; m, mitochondrion; pm, plasma membrane; tz, transition zone. Bar, 0.4 µM.