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Fig. 9. Phosphorylation of purified POK-2 and centrin. Overexpressed CaM (POK-2) and engineered centrin of Chlamydomonas were purified and prepared for phosphorylation by CaPK-1 and CaPK-2. Lanes 1 and 2, 2 µg POK-2; lanes 3 and 4, 1 µg centrin. Autophosphorylation of each kinase and the phosphorylation of POK-2 and centrin are indicated.