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Fig. 6. Adhesion-mediated activation of HA-SGK1 is not blocked by Rac1N17. MDCK cells were cotransfected with HA-SGK1 and myc-Rac1V12 or myc-Rac1N17, allowed to adhere to fibronectin and the activation of HA-SGK1 was measured as described in Fig. 1. The lower insert depicts the levels of HA-SGK1, Rac1N17 and Rac1V12 present in the assay.