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Fig. 7. Deattachment-induced FKHRL1 dephosphorylation is prevented by activated SGK1. MDCK cells were transfected with activated SGK1(S/D) (lanes 1, 2, 5 and 6) or empty vector (pKH3) (lanes 3, 4, 7 and 8) and serum starved overnight. Cells were deattached by trpysin incubation and kept in suspension for 1 hour (1 hour) or lysed immediately (0 hours). Duplicated cell extracts were prepared and analyzed for the presence of phosphorylated FKRHL1 (T32) (top panel), total FKRHL1 (middle panel) or for the presence of HA-SGK1 (bottom panel)