Fig. 2. Inhibition of P2 receptors increased osmotic fragility. Cells were incubated for 1 minute in isosmotic Ringer solution with hexokinase (hexo, 2.5 U/ml, n=10) or suramin (sur, 100 µM, n=10) prior to dilution. The control was diluted (0.5x) amphibian Ringer. Gramicidin (gram, 5 µM, n=10) and A23187 (A23, 0.5 µM, n=10) were used in the presence of either hexokinase or suramin. Values are means±s.e.m of optical density. ^**P<0.01, ^***P<0.001.