Fig. 6. Calcium ionophore potentiated cell volume recovery following hypotonic shock. At time 0, cells were abruptly exposed to a hypotonic (0.5x) amphibian Ringer solution. Cell volume recovery was enhanced with A23187 (0.5 µM, n=8), regardless of whether it was added to the extracellular medium at 5, 40 and 70 minutes after hypotonic challenge (indicated by arrows, P<0.05 compared to control values immediately following addition of ionophore, regardless of when it was added). Values are means±s.e.m.