Fig. 7. An ATP scavenger inhibited cell volume recovery following hypotonic shock. At time 0, cells were abruptly exposed to a hypotonic (0.5x) amphibian Ringer solution. The rate of cell volume recovery was reduced with hexokinase (hexo, 2.5 U/ml, n=8), regardless of whether it was added to the extracellular medium at 5, 40 and 70 minutes after hypotonic challenge (indicated by arrows, P<0.05 compared to the control immediately following addition of enzyme at the 5 minute mark, and P<0.05 after 10 and 5 minutes when hexokinase was added at the 70 and 40 minute marks, respectively. There was no significant difference between the three hexokinase groups once this enzyme exhibited an inhibitory effect that was significantly different from control values.). Values are means±s.e.m.