JCS -- Matheos et al. 116 (1): 111 Figure IG5

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Fig. 5. Quantification of DNA abundance in the DHFR oriß region by real-time PCR. (A) Map of the DHFR initiation zone, containing the ß, ß' and ${gamma}$ initiation sites and encompassing the DHFR and 2BE2121 genes. The arrows represent the location of the amplification product using a primer set within the oriß region. (B) Quantitative realtime PCR using the LightCycler instrument, using a primer set within the oriß region or $~$ 17 kb downstream from the DHFR gene (AF028017), with DNA template extracted from the immunoprecipitation with anti-Ku70, anti-Ku86, anti-clone 162 antibodies or NGS from crosslinked or untreated CHO K1 or xrs-5 cells. Each bar represents two experiments, and one standard deviation is indicated.