Fig. 1. Phenotypes of a mob2 deletion mutant. (A) Spores from strain YDM679 that contained wild-type or deleted mob2 were cultured in YE medium and incubated overnight at 30°C. Phase-contrast images are shown. (B-G) Immunofluorescence of actin and microtubule cytoskeletons. The mob2 shut-off strain YDM1032 was grown to exponential phase at 30°C in the absence of thiamine (B-C), and thiamine (5 µg/ml) was then added and samples were taken at 15 hours (D-E) and 22 hours (F-G) after thiamine addition. Cells were fixed and then stained with actin or tubulin (MTs) antibodies.