Fig. 3. Localization of Mob2p at the cell periphery/cytoplasm and the division site. (A) Mob2p-GFP localization. Cells expressing Mob2p-GFP (YDM1203) were grown to exponential phase at 25°C. GFP fluorescence and DIC images of living cells are shown. (B) The Mob2p-13Myc level remains unchanged throughout the cell cycle. mob2-13myc cdc25-22 cells (YDM933) were synchronized by incubation at 36°C for 4 hours and then shifted to 25°C. Samples were taken at indicated time points and analyzed by western blotting with anti-Myc and anti-PSTAIRE antibodies. Cdc2p, recognized by PSTAIRE antibodies, was used as a loading control. At each time point, the percentage of anaphase and septated cells is also indicated.