Fig. 1. Structural similarity and phylogenetic relationship of Tc10 and RhoT. (A) Comparison of the amino-acid sequences of mouse Tc10 and RhoT with those of representative Rho family members. The origins of these Rho family proteins are mouse Cdc42 (accession number L78075), human RhoG (X61587), mouse Rac1(X57277), mouse RhoA (AF014371), human RhoE (X95282), human Rnd1 (Y07923), mouse RhoD (D89821) and human RhoH (Z35227). The amino-acid sequences were aligned by the method of Lipman and Pearson (Lipman and Pearson, 1985) and by eye. Amino acids at positions of >50% identity are shown in red. G1-G4, core motifs for GTPase activity and GTP/GDP-binding. E, effector domain. Switch I, switch II, Rho insert regions and CaaX motif are also indicated. (B) Phylogenetic relationship of Rho family proteins. The phylogenetic tree was drawn by using TreeView (Page, 1996). Rho family proteins are subdivided into six subfamilies. RhoT and Tc10 belong to the Cdc42 subfamily. (C) Comparison of the cDNA sequences of mouse RhoT (AB060651) and TCL (AJ276568) with the corresponding mouse genomic sequence of chromosome 12, which was searched using the Ensembl Genome Browser. Only a portion containing the initiation codon and corresponding amino-acid sequences of RhoT and TCL are shown. Matched nucleotides are marked with asterisks. Gaps are indicated with dashes.