Fig. 2. Expression of Cdc42, Tc10 and RhoT in several tissues and cells during differentiation. (A) Northern blots showing the expression of Cdc42 (a), Tc10 (b) and RhoT (c). (d) Ethidium bromide staining pattern of the agarose gel electrophoresis of the total or cytoplasmic RNAs showing the 28S and 18S rRNAs. The RNAs were derived from newborn mouse brain and adult mouse tissues; C2 cells during differentiation and 10T1/2 cells; PC12 cells during differentiation; and N1E-115 cells during differentiation. C2 myoblasts (Mb) were left in the differentiation medium for the time (hours) indicated in the figure and differentiated to form myotubes (Mt) by 96 hours. Differentiation of PC12 cells was induced with 0.5 mM dbcAMP or with 50 ng/ml NGF. Differentiation of N1E-115 cells was induced by serum starvation [serum (-)] or with 2% DMSO. The time (hours) after the treatment with each reagent is noted. The size (kb) of each mRNA band is designated at the left of the panel. The positions of 28S and 18S rRNAs are indicated at the right. Two faint bands with slower mobility than the 2.5 kb RhoT band in the (c) leftmost panel are unerased Tc10 bands after rehybridization. (B) Quantitative RT-PCR analyses for the expression of RhoT (a) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a control (b) in brain and PC12 and N1E-115 cells. PC12 and N1E-115 cells were treated with NGF (N) or dbcAMP (A) and by serum starvation (S) or with DMSO (D) for 96 hours to induce differentiation.