Fig. 4. Generation of neurites by Tc10 or RhoT but not by Cdc42. PC12 cells (A,C) and N1E-115 cells (B,D) were transfected with the cDNAs of wt or constitutively active forms of Cdc42, Tc10 and RhoT fused to pEF-BOS/Myc vector. (A,B) Fluorescent micrographs of the cells expressing the Myc-tagged proteins. Forty-eight hours after transfection, the cells were stained with Myc1-9E10 to detect wt (a,c,e) and constitutively active (b,d,f) forms of Cdc42 (a,b), Tc10 (c,d) and RhoT (e,f). Bar, 20 µm. (C,D) Ratio of the neurite-extending cells. The degree of neurite extension is expressed as a multiple of the cell body diameter as indicated in the legends. More than 100 cells were assessed in each experiment. The values are the means±s.d. of triplicate experiments.