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Fig. 4. Bundling of F-actin filaments by the 34 kDa {Delta}EF2 protein is not inhibited by calcium. Mixtures of F-actin and the 34 kDa proteins in the presence of low and high concentrations of calcium were viewed by transmission electron microscopy using negative staining. Top row, wild-type 34 kDa protein and F-actin at low (A) and high (B) concentrations of calcium. Bottom row, 34 kDa {Delta}EF2 protein and F-actin at low (C) and high (D) concentrations of calcium. Mixtures of the wild-type 34 kDa protein and F-actin form bundles in the presence of low but not high concentrations of calcium. By contrast, mixtures of 34 kDa {Delta}EF2 protein and F-actin form bundles that are not calcium regulated. Magnification 14,000x.