Fig. 6. Growth curves in 34 kDa/-actinin double null amoebae rescued with 34 kDa and 34 kDa EF2 proteins. Cells were grown axenically in shaking cultures, and cell density was measured as a function of time after inoculation. (A) 20°C, (B) 15°C, AX2 (), 34 kDa/-actinin double null (), r34 kDa (), r34 kDa EF2 ([UNK]). The 34 kDa/-actinin double null cells grow more slowly than wild-type at both 20°C and 15°C. Expression of the wild-type 34 kDa protein restores wild-type growth. By contrast, expression of 34 kDa EF2 protein stimulates growth only slightly at 20°C (A) and has no restorative effect on growth of the 34 kDa/-actinin double mutant at 15°C (B).