Fig. 6. The mitotic phenotypes of Op18-NR-helix derivatives. KA8 cells were transfected and hygromycin-resistant cell lines were selected and induced with Cd²⁺ as in Fig. 3. After 24 hours, cells were fixed and double stained with anti--tubulin (green color) and propidium iodide DNA staining (red color). Representative examples of normal and abnormal metaphase cells observed using epifluorescence are shown. Type I mitotic figures lack most, but not all, kinetochore MTs and condensed chromosomes appeared unorganized. Type II mitotic figures completely lack kinetochore MTs, and condensed chromosomes appeared unorganized and in some cases even aggregated. MTs appeared as small star-like asters with dense but short MTs. Most cells contained two asters, suggesting that the centrosomes have separated.