Fig. 6. Properties of intercellular channels formed from Cx43.4 are similar to Cx45. N2A cells were transiently transfected with Xenopus Cx43.4 and analyzed by dual whole-cell voltage clamping. (A) Time-dependent inactivation of junctional currents in response to transjunctional potentials; 5 seconds long, 20 mV steps between -100 and +100 mV are shown. (B) Normalized steady-state conductance plotted against transjunctional voltage closely matches a double Boltzman distribution (N=7; V0=20 mV; Gmin/Gmax=0.05). (C) Single channel recordings display 35 pS transitions.