Fig. 6. MTIP is progressively lost during development of hepatic stages, reflecting the disassembly of the inner membrane complex (IMC). (A) Early hepatic trophozoite stage showing a typical transformation bulb. Heat shock protein 70, which is not significantly expressed in sporozoites shows increase of expression. (B) A spherical hepatic trophozoite (24 hours after invasion) shows still complete circumferential MTIP staining. (C,D) Later stage hepatic stages (36-45 hours after invasion) show progressive loss of MTIP staining closely resembling the progressive loss of the IMC during these stages of development (Meis et al., 1985). DIC, differential interference contrast.