Fig. 4. Effect of EhPAK on E.histolytica morphology. The histograms represent the number of pseudopod counted for each strain. Fixed amoebae were examined by microscopy, and pseudopodia (indicated by the arrow) and/or pseudopodia-like structures (indicated by the stars) were counted as described in Materials and Methods. Note that the majority of control cells (90%) showed one to three pseudopodia. By contrast, 90% of the C-PAK⁺ strain simultaneously displayed three to six protrusions.