Fig. 5. Time-lapse confocal imaging of vesicles containing GFP-insulin in 3T3L1 adipocytes. 3T3L1 adipocytes were infected with Adex1CA pchi-GFP, 2 days later, time-lapse confocal imaging was performed. (A) Sequential images of 3T3L1 adipocytes expressing insulin-GFP. 3T3L1 adipocytes were stimulated by 10^-7 M insulin, and each image was acquired every minute by setting the confocality to 5 µm. Typical confocal images were represented. (B) Change in the total number of GFP-labeled vesicles during stimulation was plotted against time (n=5 cells). The bars were expressed as a percentage of initial count of vesicle number prior to insulin stimulation.