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Fig. 6. Glucose uptake by 3T3L1 adipocytes. 3T3L1 adipocytes were infected with either Adex1CA pchi or Adex1w (for control), 2 days later, cells were preincubated for 1 hour under Hanks' solution without insulin and treated with different concentrations of insulin for 30 minutes at 37°C. Then, glucose uptake assay was performed over 20 minutes at room temperature per 1x106 cells under 10-9 M~10-7 M insulin using 14C-2deoxy-D-glucose as a tracer (n=4). Data are means±s.e.m.