Fig. 5. Prevention of the synthesis of the SR Ca²⁺ATPase by the anti-ß-subunit antibody. Oocytes were injected with cRNA for the SR Ca²⁺ATPase (10 ng oocyte^–1) and incubated in modified Barth's medium at 19°C. After incubation for 10 hours, the oocytes were injected with varying volumes of antibody, adjusted to a total volume of injection at 23 nl with water and allowed to stand for 60 minutes. The volumes of antibody injected were 0 nl, 0.08 nl, 0.23 nl, 0.77 nl, 2.3 nl, 7.7 nl and 23 nl (from left to right). The oocytes were then labeled with [³⁵S]methionine plus [³⁵S]cysteine for 30 minutes, followed by immunoprecipitation with anti-Ca²⁺-ATPase antiserum.