Fig. 13. Cells stained for actin (red), vinculin (green) and the nucleus (blue) as described in Materials and Methods. (A) Cell on a substrate with 600 nm deep grooves and 70 nm wide ridges on a 400 nm pitch. (B) Cell on a substrate with 600 nm deep grooves and 1900 nm ridges on a 4000 nm pitch. A reflection image of the substrates is included in the figure insets. (C) Cell on a smooth silicon oxide substrate.