Fig. 6. HGF/SF effects on AIF nuclear distribution and large scale DNA fragmentation. Nuclear extracts were analyzed by western blotting for AIF (top) obtained 6 hours following either no treatment or treatment with HGF/SF (50 ng/ml for 48 hours) and menadione (50 µM for 2 hours). The blot was stripped and re-blotted with anti-Oct-1 antibody (middle) to normalize for protein loading. Pulse field gel electrophoresis was performed (as described in Materials and Methods) on 10 µg of genomic DNA from ARPE-19 cells exposed to similar conditions (bottom).