Fig. 3. Qualitative assessment of neurite outgrowth from SH-SY5Y cells plated on purified laminin-1. Cells were either not exposed to adenovirus (A) or exposed to adenovirus expressing only green fluorescent protein (GFP, B, and corresponding fluorescent image C), co-expressing GFP and wildtype (WT-Rit, D, and corresponding fluorescent image E) or co-expressing GFP and constitutively active Rit (CA-Rit, F, and corresponding fluorescent image G). SH-SY5Y cells expressing WT-Rit or CA-Rit have well developed growth cones (arrowheads in D,F) and display a qualitative increase in neurite outgrowth including marked increases in branching (arrows in F). Bar, 50 µm.