Fig. 1. Two-hybrid analysis of the amphiphysin 2/SNX4 interaction. (A) Different portions of human SNX4 cDNA cloned in the pGAD vector were co-expressed in the L40 yeast strain with full length or partial Amp2m (residues 1-304) cloned in the pLex vector. The three upper fragments correspond to clones selected in the two-hybrid screen when the three lower fragments were cloned secondarily. (B) Different portions of mouse Amp2m cDNA cloned in the pLex vector were co-expressed in the L40 yeast strain with a C-terminal part of human SNX4 (residues 377-450 or 408-450) cloned in the pGAD vector. In both cases, yeast cells were selected on medium deficient in tryptophan and leucine. Protein interaction was analyzed in a beta-galactosidase assay.