Fig. 4. Comparison of the distribution of SNX4 with markers of intracellular organelles. HeLa cells were transiently transfected with SNX4 in pRK5myc, fixed with PFA and stained with relevant antibodies: the anti-myc mouse monoclonal antibody and antibodies specific for markers of intracellular organelles, anti-EEA1 (early endosomes), anti-Rab11 (recycling endosomes), anti-CD63 (late endosomes/lysosomes), anti-LAMP1 (lysosomes), and anti-calnexin (endoplasmic reticulum). In the case of GFP-Rab5, cells were co-transfected with SNX4 in pRK5myc and GFP-Rab5 before staining with anti-myc antibody. Images were acquired on a confocal microscope and pseudo-colored with Metamorph. Co-localization appears in yellow.