Fig. 5. Comparison of the distribution of amphiphysin 2 with markers of intracellular organelles. HeLa cells were transiently transfected with Bramp2 in pRK5myc, pre-permeabilized in 0.01% saponin and fixed with PFA. Then, they were stained for myc expression and organelle-specific markers: anti-EEA1 (early endosomes), anti-CD63 (late endosomes/lysosomes), anti-LAMP1 (lysosomes), and anti-calnexin (endoplasmic reticulum). In the case of GFP-Rab11, cells were co-transfected with Bramp2 in pRK5myc and GFP-Rab11 before staining with anti-myc antibody. Images were acquired on a confocal microscope and pseudo-colored with Metamorph. Colocalization appears in yellow.