Fig. 6. Effect of SNX4 overexpression on transferrin receptor endocytosis. HeLa cells were transfected with SH3-Amp2 in pRK5myc, with a fragment of Amp2 (residues 42-147) in pRK5myc, with full length SNX4 in pRK5myc or with C-terminal SNX4 in pRK5myc (residues 368-450). Cells were incubated with Alexa 488-transferrin for 15 minutes at 37°C before PFA fixation and staining with anti-myc antibody followed by Cy3-coupled anti-mouse antibody. Images were acquired on a confocal microscope and pseudo-colored with Metamorph.