Fig. 2. The C-terminal extremity of TACE is involved in the binding with SAP97. (A) Interaction analyzed by yeast two-hybrid system. Full-length SAP97 and mutant forms of TACE were expressed as fusion proteins with a GAL4 activation domain and a GAL4 DNA-binding domain, respectively (see Materials and Methods) and are schematically represented in the lower part of the figure. (B) Western blot showing that TACE mutant forms fused to GAL4 DNA-binding domain (GAL4BD) are expressed in yeast. Detection was made using anti-GAL4BD monoclonal antibody.