JCS -- Peiretti et al. 116 (10): 1949 Figure IG4

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 4. Coexpression of TACE and SAP97 alters their cellular distribution. COS-7 cells were transfected with different forms of haTACE and GFP-SAP97 and their cellular distribution was analyzed. (A) GFP-SAP97 presents a distribution similar to that of GFP-SAP97_D_PDZ3. (B) haTACE presents a distribution similar to that of haTACEm. (C) Distribution of GFP-SAP97 when coexpressed with haTACE (three representative cellular distributions are shown). (D) Distribution of GFP-SAP97 when coexpressed with haTACEm. (E) Distribution of GFP-SAP97_D_PDZ3 when coexpressed with haTACE. Distribution of GFP-SAP97 overexpressed forms were assessed by GFP epifluorescence with a FITC filter. TACE overexpressed forms were detected using anti-HA epitope antibodies followed by Rhodamine-conjugated secondary antibodies and their distributions revealed by Rhodamine epifluorescence.