Fig. 7. Endogenous TACE and SAP97 can colocalize. (A) Immunocytochemistry for the detection of endogenous TACE and SAP97 was performed, as described in Materials and Methods, in confluent COS-7 cells and CACO-2 cells. The localization was analyzed by immunofluorescence confocal microscopy using separate channels. TACE: localization of endogenous TACE. SAP97: localization of endogenous SAP97. TACE+SAP97: superimposition of the two previous images. Areas where TACE and SAP97 colocalized appeared in a deep yellow color. (B) Western blots of CACO-2 cells lysate. Lane 1 detection of TACE; lane 2 detection of SAP97. Sizes in kDa are indicated on the left.