Fig. 2. Effects of CaM antagonists on tyrosine phosphorylation of the mouse sperm components. Spermatozoa were incubated for 90 minutes at 37°C in EKRB medium supplemented with 3 mg BSA/ml (A) or 1 mM methyl-ß-cyclodextrin (B) in the absence or presence of different concentrations (µM) of CaM antagonists (W7, 25; OA, 20; CZ, 2.5). The lanes with solvent alone (water, methanol and DMSO) are controls for each antagonist. Tyrosine phosphorylation of the sperm components was revealed as above. The figure shows results of a representative experiment. Similar results were obtained in two other experiments.